4JNH
A unique spumavirus gag N-terminal domain with functional properties of orthoretroviral Matrix and Capsid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.423, 94.729, 61.659 |
| Unit cell angles | 90.00, 93.68, 90.00 |
Refinement procedure
| Resolution | 23.682 - 2.402 |
| R-factor | 0.1749 |
| Rwork | 0.172 |
| R-free | 0.22980 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.044 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.160 | 2.400 |
| Rmerge | 0.061 | 0.049 | 0.110 |
| <I/σ(I)> | 12 | ||
| Completeness [%] | 98.1 | 97.1 | 95.8 |
| Redundancy | 3.2 | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 289 | 10mg/ml solution of PFV-Gag-NtD in 150mM NaCl, 5% glycerol, 10mM Tris-HCl, pH 8.0 was mixed with an equal volume of crystallisation solution containing 16% PEG 6000 (w/v), 12% ethylene glycol, 0.03M MgCl2 hexahydrate , VAPOR DIFFUSION, temperature 289K |
