4JN9
Crystal structure of the DepH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-20 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97916 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 78.120, 78.120, 187.415 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.518 - 1.900 |
R-factor | 0.1642 |
Rwork | 0.162 |
R-free | 0.19680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fbs |
RMSD bond length | 0.007 |
RMSD bond angle | 1.124 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.113 | 0.043 | 0.627 |
Number of reflections | 53334 | ||
<I/σ(I)> | 6.9 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 11.1 | 10.5 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M citric acid, 0.2M (NH4)2SO4, 11.75 % PEG4000, microseeding, temperature 293K, pH 5.0, VAPOR DIFFUSION, HANGING DROP |