4JN9

Crystal structure of the DepH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-20
Detector	RAYONIX MX-225
Wavelength(s)	0.97916
Spacegroup name	P 31 2 1
Unit cell lengths	78.120, 78.120, 187.415
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.518 - 1.900
R-factor	0.1642
Rwork	0.162
R-free	0.19680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fbs
RMSD bond length	0.007
RMSD bond angle	1.124
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.113	0.043	0.627
Number of reflections	53334
<I/σ(I)>	6.9
Completeness [%]	100.0	99.8	100
Redundancy	11.1	10.5	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1 M citric acid, 0.2M (NH4)2SO4, 11.75 % PEG4000, microseeding, temperature 293K, pH 5.0, VAPOR DIFFUSION, HANGING DROP