4JN5
Crystal structures of the first condensation domain of the CDA synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-02-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.130, 82.438, 87.732 |
| Unit cell angles | 90.00, 106.34, 90.00 |
Refinement procedure
| Resolution | 33.905 - 2.440 |
| R-factor | 0.2036 |
| Rwork | 0.200 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.497 |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.440 |
| Number of reflections | 31620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 9-13% PEG 10000, 0.1 M HEPES, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
