4JN3
Crystal structures of the first condensation domain of the CDA synthetase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-24 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97860, 0.9792, 0.9070 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.789, 83.479, 177.589 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.112 - 1.690 |
R-factor | 0.1928 |
Rwork | 0.192 |
R-free | 0.21200 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.821 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.690 |
Number of reflections | 100050 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 25-27% PEG 3000, 0.2-0.25M lithium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |