4JN3
Crystal structures of the first condensation domain of the CDA synthetase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97860, 0.9792, 0.9070 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.789, 83.479, 177.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.112 - 1.690 |
| R-factor | 0.1928 |
| Rwork | 0.192 |
| R-free | 0.21200 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.821 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.690 |
| Number of reflections | 100050 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 25-27% PEG 3000, 0.2-0.25M lithium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
