4JMQ
Crystal structure of pb9: The Dit of bacteriophage T5.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2011-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.657, 70.027, 71.014 |
| Unit cell angles | 91.05, 107.66, 112.03 |
Refinement procedure
| Resolution | 27.689 - 1.895 |
| R-factor | 0.2053 |
| Rwork | 0.203 |
| R-free | 0.25180 |
| Structure solution method | SAD |
| Starting model (for MR) | Poly-Ala model |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.139 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.689 |
| High resolution limit [Å] | 1.890 |
| Rmerge | 0.159 |
| Number of reflections | 70953 |
| <I/σ(I)> | 6.38 |
| Completeness [%] | 95.4 |
| Redundancy | 2.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 10 to 14% (w/v) PEG 3350, 0.05M MES, 0.1 to 0.2M MgCl2, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
