4JMP

Crystal structure of the chimerical protein CapA2B2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-31
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 1 21 1
Unit cell lengths	36.332, 88.666, 39.405
Unit cell angles	90.00, 115.35, 90.00

Refinement procedure

Resolution	33.046 - 1.300
R-factor	0.1726
Rwork	0.172
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bfv
RMSD bond length	0.006
RMSD bond angle	1.012
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.600	1.370
High resolution limit [Å]	1.300	1.300
Rmerge		0.551
Number of reflections	54294
<I/σ(I)>		2.1
Completeness [%]	98.3	99.6
Redundancy		2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	290	25% PEG550 MME, 100mM Hepes, 5mM MgCl2, pH 7.5, VAPOR DIFFUSION, temperature 290K