4JM0
Structure of Human Cytomegalovirus Immune Modulator UL141
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-03 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 96.060, 96.060, 136.071 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 11.980 - 3.250 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 2.352 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0104) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 11.980 | 3.330 |
High resolution limit [Å] | 3.250 | 3.250 |
Number of reflections | 10665 | |
Completeness [%] | 75.0 | 97 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 295.15 | 0.2 M calcium acetate, 0.1 M imidazole pH 8, 10% (w/v) polyethylene glycol 8000, VAPOR DIFFUSION, SITTING DROP, temperature 295.15K |