4JLY
Dodecameric structure of spermidine N-acetyltransferase from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 194.860, 71.771, 136.728 |
| Unit cell angles | 90.00, 134.53, 90.00 |
Refinement procedure
| Resolution | 28.910 - 2.882 |
| R-factor | 0.18973 |
| Rwork | 0.185 |
| R-free | 0.27698 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3EG7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.557 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Rmerge | 0.076 | 0.720 |
| Number of reflections | 30456 | |
| <I/σ(I)> | 22.03 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05 M Ammonium Sulfate, 0.1 M tri-Sodium Citrate, 15 % PEG8000 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
