4JLW
Crystal structure of formaldehyde dehydrogenase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.226, 98.305, 99.623 |
| Unit cell angles | 90.00, 91.29, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.2118 |
| Rwork | 0.209 |
| R-free | 0.25936 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kol |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.641 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.203 | 0.634 |
| Number of reflections | 47323 | |
| <I/σ(I)> | 6.43 | 2.91 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.1M Bis-Tris, 1.8M ammonium sulfate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
