4JLE

Structure of the P. falciparum PFI1780w PHIST domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 31 2 1
Unit cell lengths	71.030, 71.030, 146.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.710 - 2.350
R-factor	0.2092
Rwork	0.207
R-free	0.25530
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	0.960
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	146.400	2.480
High resolution limit [Å]	2.350	2.350
Rmerge	0.063	0.494
Number of reflections	18438
<I/σ(I)>	11.8	2.4
Completeness [%]	99.9	100
Redundancy	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	2.0M Sodium Chloride 0.1M Sodium Acetate 22.5% Glycerol, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K