4JLC
Crystal structure of mouse TBK1 bound to SU6668
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2013-01-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 140.480, 140.480, 86.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.530 - 3.000 |
| R-factor | 0.2368 |
| Rwork | 0.234 |
| R-free | 0.26690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4EUT AND 3QA8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.826 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.530 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.106 | |
| Number of reflections | 20027 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1 M Sodium Citrate, 0.1 M HEPES, 10-14% isopropanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
