4JL0
Crystal structure of PcrH in complex with the chaperone binding region of PopB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.977960 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 45.100, 73.290, 98.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.300 - 2.220 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.26480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xcc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.069 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.300 | 2.390 | |
| High resolution limit [Å] | 2.220 | 6.690 | 2.250 |
| Rmerge | 0.047 | 0.017 | 0.415 |
| Number of reflections | 16247 | 704 | 2560 |
| <I/σ(I)> | 29.7 | 79.82 | 4.78 |
| Completeness [%] | 99.4 | 98.3 | 96.8 |
| Redundancy | 6.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 30% PEG6000, 1.0M LiCl/ 0.1 M tris-HCl pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
