4JKX

Crystal structure Mistletoe Lectin I from Viscum album in complex with kinetin at 2.35 A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	OTHER
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-05
Detector	PSI PILATUS 6M
Wavelength(s)	0.826
Spacegroup name	P 65 2 2
Unit cell lengths	107.010, 107.010, 312.420
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	79.700 - 2.350
R-factor	0.22179
Rwork	0.220
R-free	0.24822
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eb2
RMSD bond length	0.009
RMSD bond angle	1.320
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.700		2.400
High resolution limit [Å]	2.350	10.000	2.350
Rmerge	0.082	0.013	0.013
<I/σ(I)>	14.09	53.64	1.11
Completeness [%]	99.9	98	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	2.5	293	1.0M ammonium sulphate, 0.2M glycine/HCl, pH 2.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K