4JKQ

Crystal structure of the N-terminal region of the human ryanodine receptor 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.918
Spacegroup name	P 42 21 2
Unit cell lengths	75.450, 75.450, 248.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.770 - 2.390
R-factor	0.2258
Rwork	0.224
R-free	0.26087
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xoa
RMSD bond length	0.008
RMSD bond angle	1.185
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	124.420	2.520
High resolution limit [Å]	2.390	2.390
Rmerge	0.160
Number of reflections	29469
<I/σ(I)>	12.2
Completeness [%]	100.0	95
Redundancy	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	20 mM TRIS-HCl, 150 mM NaCl, 10% glycerol, 7mM mercaptoethanol, 1 mM CHAPS, 0.1% betaine, 100 mM HEPES, 200 mM ammonium formate, 21% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K