4JKM

Crystal Structure of Clostridium perfringens beta-glucuronidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-08-03
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	71.511, 292.612, 239.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.791 - 2.263
R-factor	0.2081
Rwork	0.207
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.832
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.770
High resolution limit [Å]	2.260
Rmerge	0.098
Number of reflections	117154
<I/σ(I)>	12
Completeness [%]	99.6
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.1 M MES pH 6.0-6.5, 28-36% PEG 400, 0.02% sodium azide, vapor diffusion, hanging drop, temperature 289.15K