4JKK
Crystal Structure of Streptococcus agalactiae beta-glucuronidase in space group I222
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-08-10 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 68.814, 83.829, 199.332 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 99.670 - 2.590 |
R-factor | 0.15 |
Rwork | 0.146 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.436 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.670 | 2.640 |
High resolution limit [Å] | 2.590 | 2.600 |
Rmerge | 0.128 | 0.363 |
Number of reflections | 18183 | |
<I/σ(I)> | 7.9 | |
Completeness [%] | 99.1 | 100 |
Redundancy | 6.6 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 289.15 | 20-28% PEG 3350, 0.1-0.3 M KSCN, 0.02% sodium azide, vapor diffusion, hanging drop, temperature 289.15K |