4JKJ
Crystal Structure of the S18Y Variant of Ubiquitin Carboxy-terminal Hydrolase L1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 109.585, 109.585, 79.715 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.208 - 2.151 |
| R-factor | 0.198 |
| Rwork | 0.197 |
| R-free | 0.24460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2etl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.075 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.920 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.710 | 2.150 |
| Number of reflections | 26890 | ||
| <I/σ(I)> | 41.4 | 22.79 | 4.05 |
| Completeness [%] | 99.9 | 100 | 99.9 |
| Redundancy | 12.5 | 12.9 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.4M Ammonium sulfate, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
