4JJQ

Crystal structure of usp7-ntd with an e2 enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 41
Unit cell lengths	69.901, 69.901, 45.733
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.430 - 1.950
R-factor	0.197
Rwork	0.197
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yy6
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.950
Number of reflections	17000
<I/σ(I)>	29

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	30 % PEG 4000, 0.1 M TRIS PH 8.5 AND 0.2 M LITHIUM SULFATE , VAPOR DIFFUSION, HANGING DROP, temperature 277K