4JIS
Crystal structure of ribitol 5-phosphate cytidylyltransferase (TarI) from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2012-03-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.747, 60.977, 91.808 |
| Unit cell angles | 90.00, 113.44, 90.00 |
Refinement procedure
| Resolution | 24.710 - 1.772 |
| R-factor | 0.2082 |
| Rwork | 0.206 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f1c |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.275 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.840 |
| High resolution limit [Å] | 1.772 | 1.780 |
| Rmerge | 0.458 | |
| Number of reflections | 49878 | |
| Completeness [%] | 97.9 | 96.8 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 20% PEG 6000, 0.1M MES-NaOH (pH 6.0), 0.2M CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
