4JI5

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	399.620, 399.620, 216.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.286 - 3.850
R-factor	0.1553
Rwork	0.153
R-free	0.20200
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.013
RMSD bond angle	1.973
Data scaling software	XSCALE
Phasing software	PHENIX (dev_1119)
Refinement software	PHENIX (dev_1119)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.950
High resolution limit [Å]	3.850	17.220	3.850
Rmerge	0.117	0.011	0.011
Number of reflections	163037	1861	12002
<I/σ(I)>	11.37	27.36	1.9
Completeness [%]	99.3	90.1	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K