4JI4

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	402.081, 402.081, 174.609
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.299 - 3.692
R-factor	0.1593
Rwork	0.156
R-free	0.21400
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.012
RMSD bond angle	1.796
Data scaling software	XSCALE
Phasing software	PHENIX (dev_1119)
Refinement software	PHENIX (dev_1119)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.910
High resolution limit [Å]	3.690	10.840	3.690
Rmerge	0.081	0.035	0.806
Number of reflections	150281	6036	23474
<I/σ(I)>	13.24	42.18	1.79
Completeness [%]	98.4	93.4	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K