4JI3

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	402.979, 402.979, 173.118
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.002 - 3.350
R-factor	0.1701
Rwork	0.168
R-free	0.21520
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	1.034
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (dev_1119)
Refinement software	PHENIX (dev_1119)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.470
High resolution limit [Å]	3.350	7.200	3.350
Rmerge	0.072	0.033	0.624
Number of reflections	178536
<I/σ(I)>	13
Completeness [%]	88.2	79.6	92.3
Redundancy	4.4	4.6	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K