4JHY

Crystal structure of a TBP-like protein (BDI_3606) from Parabacteroides distasonis ATCC 8503 at 1.90 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97939, 0.91837, 0.97895
Spacegroup name	P 1 21 1
Unit cell lengths	26.670, 82.344, 40.543
Unit cell angles	90.00, 106.83, 90.00

Refinement procedure

Resolution	28.239 - 1.900
R-factor	0.1853
Rwork	0.183
R-free	0.22610
Structure solution method	MAD
Data reduction software	XDS
Data scaling software	XSCALE (July 4, 2012)
Phasing software	SHELX
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.239		1.970
High resolution limit [Å]	1.900	4.080	1.900
Rmerge	0.060	0.032	0.358
Number of reflections	12093	2358	2127
<I/σ(I)>	7.16	16.5	1.8
Completeness [%]	84.6	88.3	80.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M ammonium chloride, 20.0% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K