4JHU
T2-depleted laccase from Coriolopsis caperata soaked with CuCl
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | KURCHATOV SNC BEAMLINE K4.4 |
| Synchrotron site | KURCHATOV SNC |
| Beamline | K4.4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.900, 84.420, 116.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.370 - 1.890 |
| R-factor | 0.16824 |
| Rwork | 0.166 |
| R-free | 0.20979 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.956 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.250 | 2.010 |
| High resolution limit [Å] | 1.890 | 2.820 | 1.890 |
| Number of reflections | 49260 | ||
| Completeness [%] | 98.0 | 99.77 | 91.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20% PEG 8000, 0.1 M HEPES, hanging drop, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
