4JHO

Structural analysis and insights into glycon specificity of the rice GH1 Os7BGlu26 beta-D-mannosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	110
Detector technology	CCD
Collection date	2009-08-21
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	68.084, 71.685, 136.675
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.490 - 2.210
R-factor	0.17841
Rwork	0.176
R-free	0.21921
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rgl
RMSD bond length	0.010
RMSD bond angle	1.155
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.490	2.990	2.280
High resolution limit [Å]	2.210	2.770	2.200
Number of reflections	34370
Completeness [%]	99.6	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.25	288	0.8M K,Na tartrate, 0.1M Na HEPES, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 288K