4JHN
The crystal structure of the RPGR RCC1-like domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9770 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.490, 118.730, 92.630 |
| Unit cell angles | 90.00, 101.54, 90.00 |
Refinement procedure
| Resolution | 29.830 - 1.700 |
| R-factor | 0.18703 |
| Rwork | 0.185 |
| R-free | 0.21835 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a12 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.986 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0093) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.830 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.323 | |
| Number of reflections | 132539 | |
| <I/σ(I)> | 5.17 | |
| Completeness [%] | 99.3 | 99.6 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M LiCl2 and 18% (w/v) PEG3350 and microseeding, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
