4JHI

Crystal Structure of Medicago truncatula Nodulin 13 (MtN13) in complex with N6-benzyladenine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-13
Detector	MAR CCD 165 mm
Wavelength(s)	1.04172
Spacegroup name	P 62 2 2
Unit cell lengths	96.086, 96.086, 113.584
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.900 - 2.600
R-factor	0.1747
Rwork	0.170
R-free	0.22540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rws
RMSD bond length	0.015
RMSD bond angle	1.614
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.900	46.900	2.760
High resolution limit [Å]	2.600	7.710	2.600
Rmerge	0.128	0.047	0.684
Number of reflections	10018	451	1568
<I/σ(I)>	17	38.05	4.33
Completeness [%]	99.8	97.8	99.6
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	292	1.7 M SODIUM MALONATE, 200 mM NaCl, 50 mM Tris-HCl, protein was incubated overnight with N6-benzylopurine prior to crystallization, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K