4JHH
Crystal Structure of Medicago truncatula Nodulin 13 (MtN13) in complex with kinetin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-12 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91801 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 96.415, 96.415, 113.407 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.220 - 2.200 |
R-factor | 0.1876 |
Rwork | 0.186 |
R-free | 0.21480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rws |
RMSD bond length | 0.020 |
RMSD bond angle | 1.725 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.220 | 48.220 | 2.330 |
High resolution limit [Å] | 2.200 | 6.540 | 2.200 |
Rmerge | 0.094 | 0.026 | 0.647 |
Number of reflections | 16292 | 721 | 2433 |
<I/σ(I)> | 14.49 | 42.19 | 1.96 |
Completeness [%] | 99.0 | 98.8 | 94.4 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 1.85 M SODIUM MALONATE, 200 mM NaCl, 50 mM Tris-HCl, protein was incubated overnight with kinetin prior to crystallization, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |