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4JHH

Crystal Structure of Medicago truncatula Nodulin 13 (MtN13) in complex with kinetin

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsBESSY BEAMLINE 14.1
Synchrotron siteBESSY
Beamline14.1
Temperature [K]100
Detector technologyCCD
Collection date2012-07-12
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)0.91801
Spacegroup nameP 62 2 2
Unit cell lengths96.415, 96.415, 113.407
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution48.220 - 2.200
R-factor0.1876
Rwork0.186
R-free0.21480
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3rws
RMSD bond length0.020
RMSD bond angle1.725
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwarePHENIX (1.8.1_1168)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]48.22048.2202.330
High resolution limit [Å]2.2006.5402.200
Rmerge0.0940.0260.647
Number of reflections162927212433
<I/σ(I)>14.4942.191.96
Completeness [%]99.098.894.4
Redundancy6.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP82921.85 M SODIUM MALONATE, 200 mM NaCl, 50 mM Tris-HCl, protein was incubated overnight with kinetin prior to crystallization, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K

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