Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

4JHH

Crystal Structure of Medicago truncatula Nodulin 13 (MtN13) in complex with kinetin

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsBESSY BEAMLINE 14.1
Synchrotron siteBESSY
Beamline14.1
Temperature [K]100
Detector technologyCCD
Collection date2012-07-12
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)0.91801
Spacegroup nameP 62 2 2
Unit cell lengths96.415, 96.415, 113.407
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution48.220 - 2.200
R-factor0.1876
Rwork0.186
R-free0.21480
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3rws
RMSD bond length0.020
RMSD bond angle1.725
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwarePHENIX (1.8.1_1168)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]48.22048.2202.330
High resolution limit [Å]2.2006.5402.200
Rmerge0.0940.0260.647
Number of reflections162927212433
<I/σ(I)>14.4942.191.96
Completeness [%]99.098.894.4
Redundancy6.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP82921.85 M SODIUM MALONATE, 200 mM NaCl, 50 mM Tris-HCl, protein was incubated overnight with kinetin prior to crystallization, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K

227111

PDB entries from 2024-11-06

PDB statisticsPDBj update infoContact PDBjnumon