4JHG

Crystal Structure of Medicago truncatula Nodulin 13 (MtN13) in complex with trans-zeatin

Replaces:  3RWS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 62 2 2
Unit cell lengths	96.110, 96.110, 113.244
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.920 - 1.850
R-factor	0.1837
Rwork	0.182
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qim
RMSD bond length	0.019
RMSD bond angle	1.578
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.920
High resolution limit [Å]	1.850	3.980	1.850
Rmerge	0.075	0.035	0.981
Number of reflections	26831
<I/σ(I)>	9.6	52.6	2.1
Completeness [%]	99.9	99.4	100
Redundancy	10.5	9.9	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	292	1.9 M SODIUM MALONATE, 200 mM NaCl, 50 mM Tris-HCl, protein was incubated overnight with trans-zeatin prior to crystallization, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K