4JHC
Crystal structure of the uncharacterized Maf protein YceF from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-26 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.413, 52.856, 128.948 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.770 - 1.850 |
| R-factor | 0.1952 |
| Rwork | 0.194 |
| R-free | 0.24670 |
| Structure solution method | 1EX2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.118 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP (11.0) |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.043 | 0.586 |
| Number of reflections | 30343 | |
| <I/σ(I)> | 37.4 | 2 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291 | 0.2M Na dihydrogen phosphate, 20% PEG3350, 1/10 papain, vapor diffusion hanging drop, temperature 291K |
