4JHB
Crystal structure of RelB double mutants: Y300F/I335F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2011-07-02 |
Detector | AGILENT ATLAS CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 65.230, 65.230, 65.870 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.450 - 2.440 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zk9 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.800 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.760 | 2.490 |
High resolution limit [Å] | 2.440 | 2.700 | 2.440 |
Rmerge | 0.087 | 0.279 | 0.657 |
Number of reflections | 5543 | ||
<I/σ(I)> | 12.4 | 7.2 | 2.8 |
Completeness [%] | 88.8 | 99 | 100 |
Redundancy | 4.4 | 4.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 30% PEG4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |