4JGX
The Structure of Sterol Carrier Protein 2 from the Yeast Yarrowia Lipolytica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-09 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 67.350, 67.350, 117.020 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.190 - 2.200 |
R-factor | 0.18815 |
Rwork | 0.187 |
R-free | 0.21714 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ikt |
RMSD bond length | 0.008 |
RMSD bond angle | 1.277 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.190 | 2.257 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 16152 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 1.4M SODIUM CITRATE, 100MM HEPES, pH 6.5, VAPOR DIFFUSION, temperature 293K |