4JGW
The conformation of a docking site for SH3 domains is pre-selected in the Guanine Nucleotide Exchange Factor Rlf
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97626 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.930, 75.620, 101.330 |
Unit cell angles | 90.00, 98.15, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 46676 | |
<I/σ(I)> | 24.3 | 3.44 |
Completeness [%] | 98.6 | 98.7 |
Redundancy | 4.73 | 3.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1M Bis-Tris propane, 0.2M NaNO3, 12% PEG 3350 , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |