4JGU
Crystal structure of Clostridium histolyticum ColH collagenase polycystic kidney-disease-like domain 2b at 1.4 Angstrom resolution in the presence of calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-03-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.387, 38.869, 54.657 |
| Unit cell angles | 90.00, 98.43, 90.00 |
Refinement procedure
| Resolution | 22.190 - 1.420 |
| R-factor | 0.15223 |
| Rwork | 0.150 |
| R-free | 0.19133 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3JQU |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.737 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 22.190 | 22.190 | 1.440 |
| High resolution limit [Å] | 1.420 | 3.060 | 1.420 |
| Number of reflections | 36663 | ||
| <I/σ(I)> | 33.1 | ||
| Completeness [%] | 96.7 | 99 | 85.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 35% (w/v) PEG 5000, 0.2M ammonium sulfate, 0.1M MES pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
