4JGR
The crystal structure of sporulation kinase D mutant sensor domain, R131A, from Bacillus subtilis subsp at 2.4A resolution
Replaces: 4DBIExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-20 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.663, 83.424, 91.006 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.920 - 2.400 |
R-factor | 0.204 |
Rwork | 0.201 |
R-free | 0.25200 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.097 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | PHENIX ((PHENIX.REFINE: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 23104 | |
<I/σ(I)> | 29.6 | 4.6 |
Completeness [%] | 99.2 | 99.7 |
Redundancy | 9.5 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2M AMMONIUM ACETATE, 0.1M BIS-TRIS AND 25% (W/V) PEG3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |