4JGR

The crystal structure of sporulation kinase D mutant sensor domain, R131A, from Bacillus subtilis subsp at 2.4A resolution

Replaces:  4DBI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-20
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	76.663, 83.424, 91.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.920 - 2.400
R-factor	0.204
Rwork	0.201
R-free	0.25200
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.097
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MLPHARE
Refinement software	PHENIX ((PHENIX.REFINE: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	23104
<I/σ(I)>	29.6	4.6
Completeness [%]	99.2	99.7
Redundancy	9.5	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	0.2M AMMONIUM ACETATE, 0.1M BIS-TRIS AND 25% (W/V) PEG3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K