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4JGR

The crystal structure of sporulation kinase D mutant sensor domain, R131A, from Bacillus subtilis subsp at 2.4A resolution

Replaces:  4DBI
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 19-BM
Synchrotron siteAPS
Beamline19-BM
Temperature [K]100
Detector technologyCCD
Collection date2011-11-20
DetectorADSC QUANTUM 315r
Spacegroup nameP 21 21 21
Unit cell lengths76.663, 83.424, 91.006
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution37.920 - 2.400
R-factor0.204
Rwork0.201
R-free0.25200
Structure solution methodSAD
RMSD bond length0.008
RMSD bond angle1.097
Data reduction softwareHKL-3000
Data scaling softwareHKL-3000
Phasing softwareMLPHARE
Refinement softwarePHENIX ((PHENIX.REFINE: 1.7.1_743))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.440
High resolution limit [Å]2.4002.400
Number of reflections23104
<I/σ(I)>29.64.6
Completeness [%]99.299.7
Redundancy9.59.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP5.52980.2M AMMONIUM ACETATE, 0.1M BIS-TRIS AND 25% (W/V) PEG3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K

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