4JGL

Crystal structure of a streptavidin-like protein (BACEGG_01519) from Bacteroides eggerthii DSM 20697 at 1.25 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.918401, 0.979338, 0.979108
Spacegroup name	P 61
Unit cell lengths	79.322, 79.322, 50.113
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.331 - 1.250
R-factor	0.135
Rwork	0.134
R-free	0.15300
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.407
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.331	28.331	1.280
High resolution limit [Å]	1.250	5.590	1.250
Rmerge		0.014	0.014
Total number of observations		6430	39805
Number of reflections	49718
<I/σ(I)>	12.6	9.1	0.5
Completeness [%]	100.0	99.1	100
Redundancy	11	10.9	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M calcium acetate, 20.0% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K