4JGI
1.5 Angstrom crystal structure of a novel cobalamin-binding protein from Desulfitobacterium hafniense DCB-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2012-05-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 31 |
| Unit cell lengths | 69.990, 69.990, 91.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.690 - 1.500 |
| R-factor | 0.14971 |
| Rwork | 0.147 |
| R-free | 0.19410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.140 |
| Data reduction software | xia2 (XDS) |
| Data scaling software | xia2 (XDS scale) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.700 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 75677 |
| Completeness [%] | 99.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277.15 | 0.1 M MMT buffer, 25 % w/v PEG 1500, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
