4JGG
Crystal Structure of TesA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.965, 103.080, 45.888 |
| Unit cell angles | 90.00, 97.57, 90.00 |
Refinement procedure
| Resolution | 40.625 - 1.900 |
| R-factor | 0.2286 |
| Rwork | 0.227 |
| R-free | 0.26230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hp4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.016 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_632)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.490 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.064 | 0.242 |
| Number of reflections | 29422 | |
| <I/σ(I)> | 13 | 4.7 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 292 | 20% PEG 3350, 50mM sodium-citrate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
