4JGE
Crystal structure of red fluorescent gene-engineered variant with improved folding - lanRFP_delS83 (Branchiostoma lanceolatum)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 133.762, 133.762, 156.956 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.760 - 1.940 |
R-factor | 0.1566 |
Rwork | 0.156 |
R-free | 0.19820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jf9 |
RMSD bond length | 0.033 |
RMSD bond angle | 2.332 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.010 |
High resolution limit [Å] | 1.940 | 4.180 | 1.940 |
Rmerge | 0.109 | 0.066 | 0.425 |
Number of reflections | 60055 | ||
<I/σ(I)> | 7.9 | ||
Completeness [%] | 97.2 | 97.6 | 89.1 |
Redundancy | 4 | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 35.6 mg/ml of lanRFP- S83 in 20 mM Tris pH 8.0, 200 mM NaCl, 5 mM EDTA mixed with an equal amount of reservoir solution - 0.2M Na2HPO4 0.1M Tris pH 7.0 20% PEG3350 , VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |