4JG5
Crystal structure of a putative cell adhesion protein (BDI_3519) from Parabacteroides distasonis ATCC 8503 at 2.34 A resolution (PSI Community Target, Nakayama)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-01-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91837,0.97853 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 60.890, 165.080, 36.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.997 - 2.340 |
R-factor | 0.1817 |
Rwork | 0.179 |
R-free | 0.23280 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.120 |
Data reduction software | XDS |
Data scaling software | XSCALE (July 4, 2012) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.997 | 49.000 | 2.420 |
High resolution limit [Å] | 2.340 | 5.020 | 2.340 |
Rmerge | 0.067 | 0.034 | 0.596 |
Number of reflections | 15739 | 1648 | 1499 |
<I/σ(I)> | 12.14 | 27.6 | 1.91 |
Completeness [%] | 96.5 | 90.9 | 98.1 |
Redundancy | 3.24 | 3.15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.29 | 277 | 28.6% polyethylene glycol 4000, 0.2M sodium acetate, 0.1M TRIS pH 8.29, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |