4JG4
Ligand concentration regulates the pathways of coupled protein folding and binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ DW |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU |
Spacegroup name | P 64 |
Unit cell lengths | 83.152, 83.152, 32.236 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.017 - 2.296 |
R-factor | 0.2161 |
Rwork | 0.213 |
R-free | 0.24920 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.488 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: dev_1111)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.020 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 5545 | |
<I/σ(I)> | 44.3 | 11.9 |
Completeness [%] | 94.4 | 78.6 |
Redundancy | 8.9 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |