4JF3

Crystal structure of the mpmv tm retroviral fusion core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-19
Detector	RIGAKU SATURN A200
Wavelength(s)	1.5418
Spacegroup name	P 3
Unit cell lengths	43.951, 43.951, 81.732
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.500 - 1.700
R-factor	0.1556
Rwork	0.154
R-free	0.19330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y4m
RMSD bond length	0.010
RMSD bond angle	1.106
Data reduction software	d*TREK (9.9.8.9D)
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.500	34.500	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.046	0.028	0.301
Total number of observations		10939	6979
Number of reflections	18731
<I/σ(I)>	19.3	50.3	4.4
Completeness [%]	96.2	99.4	85.4
Redundancy	5.41	5.58	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	18% (w/v) PEG 3400 and 0.2 M sodium thiocyanate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K