4JEO

Crystal structure of red fluorescent protein lanRFPdam exposed to prolonged X-ray irradiation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 64 2 2
Unit cell lengths	134.274, 134.274, 156.851
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.830 - 2.350
R-factor	0.1516
Rwork	0.150
R-free	0.19810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hvf
RMSD bond length	0.027
RMSD bond angle	1.993
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP (9.2)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.420
High resolution limit [Å]	2.340	5.030	2.340
Rmerge	0.131	0.043	0.627
Number of reflections	35065
<I/σ(I)>	7.2
Completeness [%]	98.7	94.5	99
Redundancy	5.6	5.5	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	9 mg/ml of lanRFP in 20 mM Tris pH 8.0, 200 mM NaCl, 5 mM EDTA mixed with an equal amount of reservoir solution - 1M Na-citrate, 0.1M Tris pH 7.0 , VAPOR DIFFUSION, HANGING DROP, temperature 293.0K