4JEL
Structure of MilB Streptomyces rimofaciens CMP N-glycosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 97.819, 97.819, 61.893 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.357 - 1.952 |
| R-factor | 0.2002 |
| Rwork | 0.198 |
| R-free | 0.24340 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.083 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 | 1.980 |
| High resolution limit [Å] | 1.950 | 2.150 | 1.950 |
| Rmerge | 0.052 | ||
| Number of reflections | 23697 | ||
| <I/σ(I)> | 40.1 | ||
| Completeness [%] | 98.2 | 97.5 | 96.9 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291 | 8% PEG 1000, 0.1 M phosphate-citrate, 0.2 M lithium sulfate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
