4JEL
Structure of MilB Streptomyces rimofaciens CMP N-glycosidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 97.819, 97.819, 61.893 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.357 - 1.952 |
R-factor | 0.2002 |
Rwork | 0.198 |
R-free | 0.24340 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.083 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 | 1.980 |
High resolution limit [Å] | 1.950 | 2.150 | 1.950 |
Rmerge | 0.052 | ||
Number of reflections | 23697 | ||
<I/σ(I)> | 40.1 | ||
Completeness [%] | 98.2 | 97.5 | 96.9 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291 | 8% PEG 1000, 0.1 M phosphate-citrate, 0.2 M lithium sulfate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |