4JEI
Nonglycosylated Yarrowia lipolytica LIP2 lipase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.931 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 116.693, 116.693, 170.272 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.970 - 2.600 |
R-factor | 0.2251 |
Rwork | 0.224 |
R-free | 0.24970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dte |
RMSD bond length | 0.008 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.730 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.120 | 56.000 |
Number of reflections | 21708 | |
<I/σ(I)> | 17.6 | 2.7 |
Completeness [%] | 96.6 | 99.5 |
Redundancy | 12 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1M Li2SO4, 2% PEG 8000 (pH 6.5)., VAPOR DIFFUSION, SITTING DROP, temperature 293K |