4JEH

Crystal Structure of Munc18a and Syntaxin1 lacking N-peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	77
Detector technology	CCD
Collection date	2007-10-21
Detector	ADSC QUANTUM 315
Spacegroup name	I 4 2 2
Unit cell lengths	154.906, 154.906, 150.324
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.640 - 2.500
R-factor	0.1867
Rwork	0.184
R-free	0.22310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c98
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	PHENIX
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.017	62.869	2.640
High resolution limit [Å]	2.500	7.910	2.500
Rmerge	0.100	0.034	0.646
Total number of observations		4753	21209
Number of reflections	31771
<I/σ(I)>	14.4	15.7	1.1
Completeness [%]	99.8	98.3	100
Redundancy	4.5	4.4	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	27% PEG 400, 10 mM EDTA, 10 mM DTT, 224 mM ammonium acetate, and 100 mM sodium acetate , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K