4JEA
Crystal structure of an engineered Zn-RIDC1 construct with four interfacial disulfide bonds
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-30 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.850, 64.788, 74.177 |
| Unit cell angles | 90.00, 98.18, 90.00 |
Refinement procedure
| Resolution | 31.940 - 1.220 |
| R-factor | 0.134 |
| Rwork | 0.131 |
| R-free | 0.17900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iq6 |
| RMSD bond length | 0.030 |
| RMSD bond angle | 3.933 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.422 | 1.280 |
| High resolution limit [Å] | 1.215 | 1.220 |
| Rmerge | 0.568 | |
| Number of reflections | 129615 | |
| <I/σ(I)> | 10.9 | 1.2 |
| Completeness [%] | 94.4 | 83 |
| Redundancy | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 293 | 35% methyl-pentanediol, 0.1M Tris pH 8.5, 0.2M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
