4JE8
Crystal structure of a human-like mitochondrial peptide deformylase in complex with Met-Ala-Ser
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Collection date | 2009-04-09 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.550, 74.710, 109.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.680 - 2.400 |
R-factor | 0.17761 |
Rwork | 0.174 |
R-free | 0.25096 |
Starting model (for MR) | 1zxz |
RMSD bond length | 0.030 |
RMSD bond angle | 2.667 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC (5.2.0019) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.400 | 2.390 |
Number of reflections | 16326 | |
<I/σ(I)> | 11.68 | 3.03 |
Completeness [%] | 99.5 | 97.2 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 5-15% PEG 5000 MME, 100mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |