4JE0
Structures of SdrD from Staphylococcus aureus reveal the molecular mechanism of how the cell surface receptors recognize their ligands
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-13 |
| Detector | MAR225 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.308, 58.363, 112.362 |
| Unit cell angles | 90.00, 111.14, 90.00 |
Refinement procedure
| Resolution | 31.440 - 1.700 |
| R-factor | 0.2104 |
| Rwork | 0.208 |
| R-free | 0.24620 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.550 | 1.710 |
| High resolution limit [Å] | 1.700 | 2.820 | 1.700 |
| Rmerge | 0.061 | ||
| Number of reflections | 85801 | ||
| <I/σ(I)> | 13.1 | ||
| Completeness [%] | 93.9 | 81.4 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.2M Calcium chloride dihydrate, 0.1M HEPES sodium(7.5) 20%(v/v) 2-Propanol , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
