4JDM
Secreted Chlamydial Protein PGP3, full-length
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930, 0.97944, 0.97818 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 188.702, 223.786, 79.152 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.123 - 3.100 |
| R-factor | 0.2331 |
| Rwork | 0.231 |
| R-free | 0.26390 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.433 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 120732 | |
| <I/σ(I)> | 12.9 | 2.1 |
| Completeness [%] | 97.3 | 90.5 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 30% PEG 550 MME, 0.1 M Tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
